Administracja Centralna Uczelni - Wymiana międzynarodowa (S2)
Sylabus przedmiotu Proteomics:
Informacje podstawowe
| Kierunek studiów | Wymiana międzynarodowa | ||
|---|---|---|---|
| Forma studiów | studia stacjonarne | Poziom | drugiego stopnia |
| Tytuł zawodowy absolwenta | |||
| Obszary studiów | — | ||
| Profil | |||
| Moduł | — | ||
| Przedmiot | Proteomics | ||
| Specjalność | przedmiot wspólny | ||
| Jednostka prowadząca | Katedra Fizjologii, Cytobiologii i Proteomiki | ||
| Nauczyciel odpowiedzialny | Agnieszka Herosimczyk <Agnieszka.Herosimczyk@zut.edu.pl> | ||
| Inni nauczyciele | Alicja Dratwa-Chałupnik <Alicja.Dratwa-Chalupnik@zut.edu.pl>, Agnieszka Herosimczyk <Agnieszka.Herosimczyk@zut.edu.pl>, Adam Lepczyński <Adam.Lepczynski@zut.edu.pl>, Katarzyna Michałek <Katarzyna.Michalek@zut.edu.pl>, Małgorzata Ożgo <Malgorzata.Ozgo@zut.edu.pl> | ||
| ECTS (planowane) | 4,0 | ECTS (formy) | 4,0 |
| Forma zaliczenia | zaliczenie | Język | angielski |
| Blok obieralny | — | Grupa obieralna | — |
Formy dydaktyczne
Wymagania wstępne
| KOD | Wymaganie wstępne |
|---|---|
| W-1 | Basic of the cell biology and the protein biochemistry. |
Cele przedmiotu
| KOD | Cel modułu/przedmiotu |
|---|---|
| C-1 | Theoretical and practical knowledge of gel-based and chromatographic protein separation techniques. |
| C-2 | The ability of the participants to use advanced bioinformatic tools to analyse proteomic data (1-D and 2-D gels, mass spectra). |
| C-3 | Practical use of MALDI-TOF MS (matrix-assisted laser desorption/ionisation time of flight mass spectrometer) for protein identification. |
Treści programowe z podziałem na formy zajęć
| KOD | Treść programowa | Godziny |
|---|---|---|
| laboratoria | ||
| T-L-1 | Sample preparation techniques for proteomic analysis. | 2 |
| T-L-2 | Protein separation using two-dimensional electrophoresis (2-DE). | 2 |
| T-L-3 | Protein separation using SDS-PAGE (1-DE). | 2 |
| T-L-4 | Protein gel staining methods. | 2 |
| T-L-5 | Identification of proteins using mass spectrometer MALTI-TOF. | 3 |
| T-L-6 | Identification of proteins using Western-blot technique. | 2 |
| T-L-7 | 1-DE and 2-DE gel image acquisition and bioinformatic analysis. | 2 |
| 15 | ||
| wykłady | ||
| T-W-1 | Introduction to proteomics. Biological significance of post-transcriptional and post-translational protein modifications. Proteome organization. The general principles of proteomic analysis. | 2 |
| T-W-2 | Gel-based protein separation techniques. The components of resolving gel matrix. Sodium-dodecyl polyacrylamide gel electrophoresis (SDS-PAGE), the principle and application of native PAGE electrophoresis. Two dimensional electrophoresis (2-DE) – the principle of the method, sample preparation for 2-DE, IPG strips, isoelectric focusing. | 2 |
| T-W-3 | Protein detection methods: coomassie stain, silver stain, negative ion staining (copper, zinc), autoradiography, fluorography, fluorescent staining. Two-dimensional difference in gel electrophoresis (2D-DIGE) – the principle and application of the method. Image acquisition and analysis of 1-D and 2-D gels. 1-D and 2-D gels analysis softwares. | 2 |
| T-W-4 | Application of mass spectrometry (MS) for protein identification. Ionization methods in mass spectrometry. Types of mass analyzers. Peptide mass fingerprinting (PMF). | 2 |
| T-W-5 | Chromatographic methods for protein separation. Liquid chromatography (LC). Two-dimensional liquid chromatography (2-D LC). The proteomic strategies based on liquid chromatography: LC-MS, LC-MS/MS, multidimensional LC-MS/MS. Affinity chromatography (AC). | 2 |
| T-W-6 | Identification of proteins using Western-blot technique. Sample preparation. Methods of protein transfer. Incubation with antibodies. Visualisation. | 3 |
| T-W-7 | Branches of proteomics: structural, functional and clinical. | 2 |
| 15 | ||
Obciążenie pracą studenta - formy aktywności
| KOD | Forma aktywności | Godziny |
|---|---|---|
| laboratoria | ||
| A-L-1 | Participation in laboratories. | 15 |
| A-L-2 | Self work with publications. | 15 |
| A-L-3 | Laboratory project preparation. | 20 |
| A-L-4 | Consultation | 10 |
| 60 | ||
| wykłady | ||
| A-W-1 | Participation in lectures. | 15 |
| A-W-2 | Study of the recommended scientific literature. | 20 |
| A-W-3 | Preparing for the writing test. | 20 |
| A-W-4 | Consultations. | 6 |
| 61 | ||
Metody nauczania / narzędzia dydaktyczne
| KOD | Metoda nauczania / narzędzie dydaktyczne |
|---|---|
| M-1 | Theoretical lectures. |
| M-2 | Discussion during laboratory classes. |
| M-3 | Project preparation. |
Sposoby oceny
| KOD | Sposób oceny |
|---|---|
| S-1 | Ocena formująca: Project presentation in the writing form. |
| S-2 | Ocena podsumowująca: Writing test. |
Zamierzone efekty kształcenia - wiedza
| Zamierzone efekty kształcenia | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|
| WM-WBiHZ_2-_null_W02 Student can enumerate and describe commonly used techniques used in the study of proteins. | — | — | C-1, C-2 | T-W-3, T-W-5, T-W-6, T-W-4, T-W-1, T-W-7, T-W-2 | M-1 | S-2 |
Zamierzone efekty kształcenia - umiejętności
| Zamierzone efekty kształcenia | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|
| WM-WBiHZ_2-_null_U01 Student is able to use commonly known proteomic techniques such as: 1-DE, 2-DE, MALDI-TOF MS and Western-blot. | — | — | C-1, C-3 | T-L-7, T-L-6, T-L-4, T-L-1, T-L-5, T-L-2, T-L-3 | M-3, M-2 | S-1 |
Zamierzone efekty kształcenia - inne kompetencje społeczne i personalne
| Zamierzone efekty kształcenia | Odniesienie do efektów kształcenia dla kierunku studiów | Odniesienie do efektów zdefiniowanych dla obszaru kształcenia | Cel przedmiotu | Treści programowe | Metody nauczania | Sposób oceny |
|---|---|---|---|---|---|---|
| WM-WBiHZ_2-_null_K01 Student is aware that there is a number of methods to analyse the different levels of protein changes in response to various physiological/patophysiological stimmuli in the biological material. | — | — | C-2, C-1, C-3 | T-L-5, T-W-6, T-L-6, T-W-2, T-W-1, T-W-4, T-W-5, T-L-3, T-W-3, T-W-7, T-L-4, T-L-1, T-L-2, T-L-7 | M-2, M-3, M-1 | S-1, S-2 |
Kryterium oceny - wiedza
| Efekt kształcenia | Ocena | Kryterium oceny |
|---|---|---|
| WM-WBiHZ_2-_null_W02 Student can enumerate and describe commonly used techniques used in the study of proteins. | 2,0 | |
| 3,0 | - student has a basic knowledge in the frame of the material program - student exerts low attitude to knowledge - in the frame of expressing knowledge student makes a lot of mistakes | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Kryterium oceny - umiejętności
| Efekt kształcenia | Ocena | Kryterium oceny |
|---|---|---|
| WM-WBiHZ_2-_null_U01 Student is able to use commonly known proteomic techniques such as: 1-DE, 2-DE, MALDI-TOF MS and Western-blot. | 2,0 | |
| 3,0 | Student, with a help of a teacher is able to meet the challenges of preparing an appropriate laboratory protocol. | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Kryterium oceny - inne kompetencje społeczne i personalne
| Efekt kształcenia | Ocena | Kryterium oceny |
|---|---|---|
| WM-WBiHZ_2-_null_K01 Student is aware that there is a number of methods to analyse the different levels of protein changes in response to various physiological/patophysiological stimmuli in the biological material. | 2,0 | |
| 3,0 | Student shows a moderate interest in participating in a verbal discussion with the teacher and colleagues during the classes. | |
| 3,5 | ||
| 4,0 | ||
| 4,5 | ||
| 5,0 |
Literatura podstawowa
- Sheehan D., Tyther R. (Ed.), Two-dimensional electrophoresis protocols., Humana Press, New York, 2009
- Garfin D., Ahuja S. (Ed.), Handbook of isoelectric focusing and proteomics., Elsevier Academic Press, Amsterdam, 2005
- Walker J.M. (Ed.), second edition, The proteomics protocols handbook., Humana Press, New Jersey, 2002
- Rabilloud T. (Ed.), .). Proteome research: two-dimensional gel electrophoresis and identification methods., Springer, Berlin, 2000
- Hames B.D. (Ed.), third edition, Gel electrophoresis of proteins: a practical approach., Oxford University Press, England, 1998