Zachodniopomorski Uniwersytet Technologiczny w Szczecinie

Wydział Nauk o Żywności i Rybactwa - Aquaculture and Fisheries (S1)

Sylabus przedmiotu Techniques of molecular biology:

Informacje podstawowe

Kierunek studiów Aquaculture and Fisheries
Forma studiów studia stacjonarne Poziom pierwszego stopnia
Tytuł zawodowy absolwenta inżynier
Obszary studiów charakterystyki PRK, kompetencje inżynierskie PRK
Profil ogólnoakademicki
Moduł —
Przedmiot Techniques of molecular biology
Specjalność przedmiot wspólny
Jednostka prowadząca Katedra Technologii Mięsa
Nauczyciel odpowiedzialny Remigiusz Panicz <rpanicz@zut.edu.pl>
Inni nauczyciele
ECTS (planowane) 5,0 ECTS (formy) 5,0
Forma zaliczenia egzamin Język angielski
Blok obieralny — Grupa obieralna —

Formy dydaktyczne

Forma dydaktycznaKODSemestrGodzinyECTSWagaZaliczenie
wykładyW6 30 3,00,50egzamin
laboratoriaL6 30 2,00,50zaliczenie

Wymagania wstępne

KODWymaganie wstępne
W-1Class in genetics and biology is recommended
W-2Students should have completed biology course. Mathematics can also be helpful to candidates in completing this course.

Cele przedmiotu

KODCel modułu/przedmiotu
C-1To provide students with a fundamental understanding of genetic principles underlying the application of molecular genetic techniques.
C-2This course is an introduction to the fundamental principles of molecular biology techniques. Students will be introduced to the study of genetics, proteins, nucleic acids and interpret primary data from current research.

Treści programowe z podziałem na formy zajęć

KODTreść programowaGodziny
laboratoria
T-L-1Safety, basic techniques, pipetting, sterile technique2
T-L-2Sampling techniques and data collection2
T-L-3Purificaton and characterisation of DNA3
T-L-4Purification and characterisation of RNA3
T-L-5Electrophoresis2
T-L-6Plan and run Polymerase Chain Reactions3
T-L-7Nested PCR2
T-L-8Extracting bands from multiplex PCR gel2
T-L-9DNA restriction2
T-L-10Absolute and relative quantification by real-time PCR4
T-L-11Gene finding, BLAST searching2
T-L-12Sequence data analysis3
30
wykłady
T-W-1Introduction and course syllabus. Nature of molecular biology. Genetic terminology.3
T-W-2DNA as the genetic material. RNA processing. Protein structure review.4
T-W-3Gene and genome structure.2
T-W-4Nucleic acid isolation, quantification and analysis.2
T-W-5Nucleic acid and protein electrophoresis2
T-W-6Polymerase Chain Reaction, and other amplification methods3
T-W-7Primer design2
T-W-8Real-time PCR2
T-W-9Introduction to cloning and sequencing2
T-W-10Molecular diagnostics. Markers.2
T-W-11Basic bioinformatics, sequence databases, sequence retrieval, alignments2
T-W-12Overview of different omics techniques2
T-W-13Questions and hypotheses of using molecular biology2
30

Obciążenie pracą studenta - formy aktywności

KODForma aktywnościGodziny
laboratoria
A-L-1Self study20
A-L-2Preparation for assessment10
A-L-3Laboratoty classes30
60
wykłady
A-W-1Class participation30
A-W-2Self-study40
A-W-3Preparaton for exam20
90

Metody nauczania / narzędzia dydaktyczne

KODMetoda nauczania / narzędzie dydaktyczne
M-1Lecture, practical classes and laboratory
M-2Lecture and laboratory
M-3Lectures
M-4Laboratory classess

Sposoby oceny

KODSposób oceny
S-1Ocena formująca: Laboratory exercises and reports
S-2Ocena podsumowująca: Exam 1 and 2
S-3Ocena formująca: Continuous assessment (laboratory)
S-4Ocena podsumowująca: Continuous assessment (laboratory)
S-5Ocena podsumowująca: Exam

Zamierzone efekty uczenia się - wiedza

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
AQF_1A_C23_W01
Uppon completion of this course students will be familiar with: - molecular tools, - bioinformatic (computation) methods, - laboratory workflow, - sampling and sample preservation.		AQF_1A_W01, AQF_1A_W03, AQF_1A_W06——C-2T-W-3, T-W-2, T-W-8, T-W-1, T-W-4, T-W-12, T-W-13, T-W-6, T-W-7, T-W-9, T-W-5, T-W-10, T-W-11M-3S-5

Zamierzone efekty uczenia się - umiejętności

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
AQF_1A_C23_U01
Uppon completion of this course the students will be able to: - perform laboratory analyses with molecular tools, - run bioinformatic calculations, - collect and preserve samples, - analyses results from molecular studies.		AQF_1A_U07, AQF_1A_U14——C-2T-L-1, T-L-2, T-L-3, T-L-4, T-L-6, T-L-5, T-L-9, T-L-10, T-L-11, T-L-7, T-L-12, T-L-8M-4S-3

Zamierzone efekty uczenia się - inne kompetencje społeczne i personalne

Zamierzone efekty uczenia sięOdniesienie do efektów kształcenia dla kierunku studiówOdniesienie do efektów zdefiniowanych dla obszaru kształceniaOdniesienie do efektów uczenia się prowadzących do uzyskania tytułu zawodowego inżynieraCel przedmiotuTreści programoweMetody nauczaniaSposób oceny
AQF_1A_C23_K01
Student will be aware to continually improve knowledge and skills.		AQF_1A_K03, AQF_1A_K05, AQF_1A_K01——C-2T-W-3, T-W-2, T-W-8, T-W-1, T-W-4, T-W-12, T-W-13, T-W-6, T-W-7, T-W-9, T-W-5, T-W-10, T-W-11, T-L-1, T-L-2, T-L-3, T-L-4, T-L-6, T-L-5, T-L-9, T-L-10, T-L-11, T-L-7, T-L-12, T-L-8M-3S-3

Kryterium oceny - wiedza

Efekt uczenia sięOcenaKryterium oceny
AQF_1A_C23_W01
Uppon completion of this course students will be familiar with: - molecular tools, - bioinformatic (computation) methods, - laboratory workflow, - sampling and sample preservation.		2,0
3,0Student is be able characterize the most common molecular tools
3,5
4,0
4,5
5,0

Kryterium oceny - umiejętności

Efekt uczenia sięOcenaKryterium oceny
AQF_1A_C23_U01
Uppon completion of this course the students will be able to: - perform laboratory analyses with molecular tools, - run bioinformatic calculations, - collect and preserve samples, - analyses results from molecular studies.		2,0
3,0Student is able to choose, prepare and applicate the proper molecular experiments
3,5
4,0
4,5
5,0

Kryterium oceny - inne kompetencje społeczne i personalne

Efekt uczenia sięOcenaKryterium oceny
AQF_1A_C23_K01
Student will be aware to continually improve knowledge and skills.		2,0
3,0Student understands at the basic level the need for continuous training and development in the field of molecular biology
3,5
4,0
4,5
5,0

Literatura podstawowa

  1. Sambrook J., Russell D.W., Molecular cloning, a laboratory manual, Cold spring harbor laboratory press, Cold spring harbor, 2001, III
  2. Green M.R., Sambrook J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 2012, 4th edition
  3. Green M.R., Sambrook J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, 2012, 4th edition
  4. Ausubel F.M. et al., Current protocols in molecular biology, John Willey & Sons, 2003

Treści programowe - laboratoria

KODTreść programowaGodziny
T-L-1Safety, basic techniques, pipetting, sterile technique2
T-L-2Sampling techniques and data collection2
T-L-3Purificaton and characterisation of DNA3
T-L-4Purification and characterisation of RNA3
T-L-5Electrophoresis2
T-L-6Plan and run Polymerase Chain Reactions3
T-L-7Nested PCR2
T-L-8Extracting bands from multiplex PCR gel2
T-L-9DNA restriction2
T-L-10Absolute and relative quantification by real-time PCR4
T-L-11Gene finding, BLAST searching2
T-L-12Sequence data analysis3
30

Treści programowe - wykłady

KODTreść programowaGodziny
T-W-1Introduction and course syllabus. Nature of molecular biology. Genetic terminology.3
T-W-2DNA as the genetic material. RNA processing. Protein structure review.4
T-W-3Gene and genome structure.2
T-W-4Nucleic acid isolation, quantification and analysis.2
T-W-5Nucleic acid and protein electrophoresis2
T-W-6Polymerase Chain Reaction, and other amplification methods3
T-W-7Primer design2
T-W-8Real-time PCR2
T-W-9Introduction to cloning and sequencing2
T-W-10Molecular diagnostics. Markers.2
T-W-11Basic bioinformatics, sequence databases, sequence retrieval, alignments2
T-W-12Overview of different omics techniques2
T-W-13Questions and hypotheses of using molecular biology2
30

Formy aktywności - laboratoria

KODForma aktywnościGodziny
A-L-1Self study20
A-L-2Preparation for assessment10
A-L-3Laboratoty classes30
60
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta

Formy aktywności - wykłady

KODForma aktywnościGodziny
A-W-1Class participation30
A-W-2Self-study40
A-W-3Preparaton for exam20
90
(*) 1 punkt ECTS, odpowiada około 30 godzinom aktywności studenta
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięAQF_1A_C23_W01Uppon completion of this course students will be familiar with: - molecular tools, - bioinformatic (computation) methods, - laboratory workflow, - sampling and sample preservation.
Odniesienie do efektów kształcenia dla kierunku studiówAQF_1A_W01Student has a basic knowledge in the field of chemistry, biochemistry, hydrochemistry, mathematics, statistics and physics.
AQF_1A_W03Student knows the statistical tools used in the engineering science.
AQF_1A_W06Students knows the terminology used in genetics and knows the basic tools of genetic engineering used in the aquatic organisms cultivation
Cel przedmiotuC-2This course is an introduction to the fundamental principles of molecular biology techniques. Students will be introduced to the study of genetics, proteins, nucleic acids and interpret primary data from current research.
Treści programoweT-W-3Gene and genome structure.
T-W-2DNA as the genetic material. RNA processing. Protein structure review.
T-W-8Real-time PCR
T-W-1Introduction and course syllabus. Nature of molecular biology. Genetic terminology.
T-W-4Nucleic acid isolation, quantification and analysis.
T-W-12Overview of different omics techniques
T-W-13Questions and hypotheses of using molecular biology
T-W-6Polymerase Chain Reaction, and other amplification methods
T-W-7Primer design
T-W-9Introduction to cloning and sequencing
T-W-5Nucleic acid and protein electrophoresis
T-W-10Molecular diagnostics. Markers.
T-W-11Basic bioinformatics, sequence databases, sequence retrieval, alignments
Metody nauczaniaM-3Lectures
Sposób ocenyS-5Ocena podsumowująca: Exam
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student is be able characterize the most common molecular tools
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięAQF_1A_C23_U01Uppon completion of this course the students will be able to: - perform laboratory analyses with molecular tools, - run bioinformatic calculations, - collect and preserve samples, - analyses results from molecular studies.
Odniesienie do efektów kształcenia dla kierunku studiówAQF_1A_U07Student is able to use computer programs supporting the accomplishment of basic engineering tasks.
AQF_1A_U14Student is able to characterise results of genetic analysis and apply basic indices in aquaculture programs, resources management and biodiversity conservation.
Cel przedmiotuC-2This course is an introduction to the fundamental principles of molecular biology techniques. Students will be introduced to the study of genetics, proteins, nucleic acids and interpret primary data from current research.
Treści programoweT-L-1Safety, basic techniques, pipetting, sterile technique
T-L-2Sampling techniques and data collection
T-L-3Purificaton and characterisation of DNA
T-L-4Purification and characterisation of RNA
T-L-6Plan and run Polymerase Chain Reactions
T-L-5Electrophoresis
T-L-9DNA restriction
T-L-10Absolute and relative quantification by real-time PCR
T-L-11Gene finding, BLAST searching
T-L-7Nested PCR
T-L-12Sequence data analysis
T-L-8Extracting bands from multiplex PCR gel
Metody nauczaniaM-4Laboratory classess
Sposób ocenyS-3Ocena formująca: Continuous assessment (laboratory)
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student is able to choose, prepare and applicate the proper molecular experiments
3,5
4,0
4,5
5,0
PoleKODZnaczenie kodu
Zamierzone efekty uczenia sięAQF_1A_C23_K01Student will be aware to continually improve knowledge and skills.
Odniesienie do efektów kształcenia dla kierunku studiówAQF_1A_K03Student is aware of the responsibility for own work and she/he ensures a compliance with the rules of the team work and takes the responsibility for the cooperative projects.
AQF_1A_K05Student is able to think and act in a creative, innovative and enterprising manner.
AQF_1A_K01Student is aware of self-knowledge and skills. Student understands the need and possibilities of continuous education and self-improvement. She/he establishes the opportunities of her/his own development and education (the second, third degree studies; postgraduate studies; courses).
Cel przedmiotuC-2This course is an introduction to the fundamental principles of molecular biology techniques. Students will be introduced to the study of genetics, proteins, nucleic acids and interpret primary data from current research.
Treści programoweT-W-3Gene and genome structure.
T-W-2DNA as the genetic material. RNA processing. Protein structure review.
T-W-8Real-time PCR
T-W-1Introduction and course syllabus. Nature of molecular biology. Genetic terminology.
T-W-4Nucleic acid isolation, quantification and analysis.
T-W-12Overview of different omics techniques
T-W-13Questions and hypotheses of using molecular biology
T-W-6Polymerase Chain Reaction, and other amplification methods
T-W-7Primer design
T-W-9Introduction to cloning and sequencing
T-W-5Nucleic acid and protein electrophoresis
T-W-10Molecular diagnostics. Markers.
T-W-11Basic bioinformatics, sequence databases, sequence retrieval, alignments
T-L-1Safety, basic techniques, pipetting, sterile technique
T-L-2Sampling techniques and data collection
T-L-3Purificaton and characterisation of DNA
T-L-4Purification and characterisation of RNA
T-L-6Plan and run Polymerase Chain Reactions
T-L-5Electrophoresis
T-L-9DNA restriction
T-L-10Absolute and relative quantification by real-time PCR
T-L-11Gene finding, BLAST searching
T-L-7Nested PCR
T-L-12Sequence data analysis
T-L-8Extracting bands from multiplex PCR gel
Metody nauczaniaM-3Lectures
Sposób ocenyS-3Ocena formująca: Continuous assessment (laboratory)
Kryteria ocenyOcenaKryterium oceny
2,0
3,0Student understands at the basic level the need for continuous training and development in the field of molecular biology
3,5
4,0
4,5
5,0